sheep anti mouse antibody  (Amersham Life Sciences Inc)

Journal: BMC Cancer

Article Title: Therapeutic efficacy of cancer stem cell-based vaccine in colorectal murine model: reduced tumor growth and prolonged survival

doi: 10.1186/s12885-026-15925-3

Figure Lengend Snippet: Immunization with CT-26 CSC and parental cell lysate induced the production of antibodies against spheroid and parental cell antigens in Group A (5 × 10 5 cells for tumor induction). I , Immunofluorescent staining of CSC lysate-vaccinated, parental cell lysate-vaccinated, and normal saline-injected mice showed immunoreactivity in fixed CT-26 spheroids. Immunofluorescent staining of parental cell-lysate-induced antibodies displayed more reactivity against CT-26 spheres. II , Immunofluorescent staining of CT-26 parental cells with sera from parental cell lysate, CSC lysate-based mice, and normal saline-injected mice revealed parental cell lysate-induced antibodies were highly reactive against parental cells. Antibody reactivity to spheroids and parental cells was visualized using FITC-conjugated anti-mouse IgG antibody and a merged image of DAPI and FITC. Immunofluorescence images are presented as representative qualitative visualizations of antibody reactivity

Article Snippet: The cells were then incubated with FITC-conjugated sheep anti-mouse IgG (Avicenna Research Institute (ARI), Tehran, Iran), diluted 1:100, at RT for an additional 45 min before being washed three times with PBS, as described previously [ ].

Techniques: Staining, Saline, Injection, Immunofluorescence

Journal: BMC Cancer

Article Title: Therapeutic efficacy of cancer stem cell-based vaccine in colorectal murine model: reduced tumor growth and prolonged survival

doi: 10.1186/s12885-026-15925-3

Figure Lengend Snippet: Immunization with CT-26 CSC and parental cell lysate induced the production of antibodies against spheroid and parental cell antigens in Group B (2.5 × 10 5 cells for tumor induction). I , Immunofluorescent staining of CSC lysate-vaccinated, parental cell lysate-vaccinated, and normal saline-injected mice showed immunoreactivity in fixed CT-26 spheroids. Immunofluorescent staining of parental cell-lysate-induced antibodies displayed more reactivity against CT-26 spheres. II , Immunofluorescent staining of CT-26 parental cells with sera from parental cell lysate, CSC lysate-based mice, and normal saline-injected mice revealed that parental cell lysate-induced antibodies were highly reactive against parental cells. Antibody reactivity to spheroids and parental cells was visualized using a FITC-conjugated anti-mouse IgG antibody and a merged image of DAPI and FITC. Immunofluorescence images are presented as representative qualitative visualizations of antibody reactivity

Article Snippet: The cells were then incubated with FITC-conjugated sheep anti-mouse IgG (Avicenna Research Institute (ARI), Tehran, Iran), diluted 1:100, at RT for an additional 45 min before being washed three times with PBS, as described previously [ ].

Techniques: Staining, Saline, Injection, Immunofluorescence